Identification of Neutralizing Epitopes within Structural Domain III of the West Nile Virus Envelope Protein
نویسندگان
چکیده
منابع مشابه
Identification of neutralizing epitopes within structural domain III of the West Nile virus envelope protein.
Using a panel of neutralizing monoclonal antibodies, we have mapped epitopes in domain III of the envelope protein of the New York strain of West Nile virus. The ability of monoclonal antibodies that recognize these epitopes to neutralize virus appeared to differ between lineage I and II West Nile virus strains, and epitopes were located on the upper surface of domain III at residues E307, E330...
متن کاملRefolding, crystallization and preliminary X-ray structural studies of the West Nile virus envelope (E) protein domain III.
Domain III of the West Nile virus envelope protein, the putative receptor-binding domain, is a major virion-surface determinant for virulence. This protein was reported to be intrinsically unstable and has defied previous crystallization attempts. It has now been purified from inclusion bodies by protein refolding and was crystallized using the hanging-drop vapour-diffusion method at 291 K. The...
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INTRODUCTION West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized agains...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 2002
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.76.24.13097-13100.2002